Yes. At verse end the prosodic continuity is broken, and the closing syllable counts as long, even if it’s a short vowel. That’s true of all meters, both Greek and Latin. Here for example is the first line of Euripides’ Bacchae (iambic trimeter): ἥκω Διὸς παῖς τήνδε Θηβαίαν χθόνα.
And the first verse of the Odyssey (dactylic hexameter): ἄνδρα μοι ἔννεπε Μοῦσα πολύτροπον ὃς μάλα πολλά.